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Monocyte function in Hodgkin's disease

G Holm, M Björkholm, B Johansson

    Clinical and Experimental Immunology
    |January 1, 1982
    PubMed
    Summary
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    Monocytes from patients with Hodgkin's disease and non-Hodgkin's lymphoma exhibit normal functions. These immune cells demonstrate typical helper, suppressor, and cytotoxic activities in patients compared to healthy individuals.

    Area of Science:

    • Immunology
    • Hematology
    • Oncology

    Background:

    • Monocytes play crucial roles in immune responses, including antibody-dependent cellular cytotoxicity and lymphocyte modulation.
    • Dysfunctional monocytes may contribute to the altered immune status observed in patients with lymphoid malignancies.

    Purpose of the Study:

    • To investigate the functional capabilities of monocytes in patients diagnosed with Hodgkin's disease (HD) and non-Hodgkin's lymphoma (NHL).
    • To assess monocyte-mediated antibody-dependent cellular cytotoxicity (ADCC) and their effects on lymphocyte proliferation and suppression.

    Main Methods:

    • Isolation and enrichment of peripheral blood monocytes (>90%) from patients with HD, NHL, and healthy controls.
    • Evaluation of monocyte ADCC using a 51Cr-release assay with anti-D IgG-coated human red blood cells (RBCs).

    Related Experiment Videos

  • Assessment of monocyte-lymphocyte interactions using concanavalin A (Con A) stimulation and measurement of 14C-thymidine incorporation.

    • Main Results:

    • Monocytes from lymphoma patients displayed normal ADCC against antibody-coated RBCs.
    • Monocytes from HD and NHL patients effectively augmented lymphocyte proliferation and suppressed responses similarly to controls.
    • Lymphocyte stimulation was generally lower in patients without added monocytes, but this difference was maintained with monocyte co-culture.

    Conclusions:

    • Monocytes from patients with active HD or NHL possess normal helper and suppressor functions on lymphocytes stimulated by Con A.
    • Antibody-dependent cytotoxicity mediated by monocytes is also normal in these patient populations.
    • These findings suggest that monocyte intrinsic functions are preserved in the context of lymphoid malignancies.