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Reverse phase high performance liquid chromatographic determination of phenprocoumon in tablets
Journal - Association of Official Analytical Chemists
|May 1, 1982
Summary
A new high-performance liquid chromatography method offers a faster and more specific way to analyze phenprocoumon in tablets. This validated assay is equivalent in accuracy and precision to the official USP method.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Phenprocoumon is an anticoagulant medication.
- Accurate and efficient assay methods are crucial for pharmaceutical quality control.
Purpose of the Study:
- To develop and validate a high-performance liquid chromatographic (HPLC) method for phenprocoumon assay in tablets.
- To compare the new method with the official United States Pharmacopeia (USP) assay.
Main Methods:
- High-performance liquid chromatography (HPLC) using an octadecylsilane (ODS) column.
- Mobile phase: 1% acetic acid in acetonitrile-water (4+3).
- UV detection at 311 nm with warfarin as an internal standard.
Main Results:
- The developed HPLC method is faster and more specific than the current USP assay.
- The method demonstrates equivalent precision and accuracy compared to the USP assay.
- Concurrent determination of phenprocoumon and potential contaminants like salicylic acid is achievable.
Conclusions:
- The new HPLC method provides a superior alternative for phenprocoumon tablet analysis.
- This method enhances efficiency and specificity in pharmaceutical quality control of phenprocoumon.