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Related Experiment Videos

Solid-phase immunoradiometric assay for C-reactive protein using magnetisable cellulose particles

F C De Beer, M B Pepys

    Journal of Immunological Methods
    |January 1, 1982
    PubMed
    Summary

    A new immunoradiometric assay (IRMA) for C-reactive protein (CRP) offers rapid and precise quantification. This method utilizes magnetic particles for efficient separation, enabling quick results for clinical diagnostics.

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    Area of Science:

    • Biochemistry
    • Immunology
    • Clinical Chemistry

    Background:

    • C-reactive protein (CRP) is a key biomarker for inflammation.
    • Accurate and rapid measurement of CRP is crucial for clinical diagnostics.
    • Existing assay methods may have limitations in speed or precision.

    Purpose of the Study:

    • To develop a novel immunoradiometric assay (IRMA) for C-reactive protein (CRP).
    • To utilize magnetisable cellulose particles for efficient solid-phase antibody support.
    • To establish a rapid and precise method for CRP quantification in clinical samples.

    Main Methods:

    • Development of an immunoradiometric assay (IRMA) using magnetisable cellulose particles coated with anti-CRP antibodies.
    • Quantification of CRP using 125I-labeled anti-CRP antibody.

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  • Rapid separation of unbound label via magnetic sedimentation and decantation.
  • Assay validation using clinical samples and comparison with electroimmunoassay.

    • Main Results:

    • The assay demonstrated a detection limit of 1 microgram CRP/l and a linear range up to 10 mg/l.
    • High throughput of 50 samples per hour with acceptable intra-assay (5.2%) and inter-assay (10.0%) coefficients of variation.
    • Excellent correlation with electroimmunoassay (r=0.975, slope=0.988) for clinical samples.
    • The assay is sensitive, precise, and rapid, suitable for high-demand clinical settings.

    Conclusions:

    • The developed magnetisable particle-based IRMA provides a sensitive, precise, and rapid method for CRP measurement.
    • The assay's efficiency in sample processing and phase separation makes it suitable for routine clinical use.
    • This novel IRMA method is a valuable tool for rapid CRP quantification, aiding in timely diagnosis and monitoring of inflammatory conditions.