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Echovirus 11 dense particles: isolation and preliminary characterization

L Cova-Baczko, M Aymard

    The Journal of General Virology
    |May 1, 1982
    PubMed
    Summary
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    Echovirus 11 produced in HeLa cells contains dense particles, differing from standard echovirus 11. These dense particles, a major component, were absent in haemagglutinating echovirus 11 from monkey kidney cells.

    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Background:

    • Echovirus 11 is a picornavirus.
    • Picornaviruses can exist in different forms, including dense particles.
    • The biological significance of dense particles in echovirus populations is not fully understood.

    Purpose of the Study:

    • To characterize dense particles of echovirus 11.
    • To investigate the conditions under which dense particles are produced.
    • To compare dense and standard echovirus 11 particles.

    Main Methods:

    • Echovirus 11 purification using Cesium Chloride (CsCl) density gradient centrifugation.
    • Characterization of viral particles by buoyant density, infectivity assays, and analysis of RNA and protein composition.

    Related Experiment Videos

  • Comparison of echovirus 11 produced in HeLa cells versus primary monkey kidney (MK) cells.

    • Main Results:

    • Dense echovirus 11 particles (density 1.44 g/ml) were isolated from CsCl gradients.
    • These dense particles shared antigenic properties and molecular composition with standard echovirus 11 (density 1.33 g/ml) but had lower infectivity and smaller diameter.
    • Dense particles constituted a major component of the echovirus 11 population from HeLa cells, which produced non-haemagglutinating virus; they were undetectable in haemagglutinating echovirus 11 from MK cells.

    Conclusions:

    • Echovirus 11 can produce dense particles as a significant population component, particularly in HeLa cells.
    • The presence of dense particles correlates with non-haemagglutinating properties and specific cell culture conditions.
    • Further research is needed to elucidate the functional role and formation mechanism of echovirus 11 dense particles.