Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantification for the x-ray microanalysis of cryosections

T A Hall, B L Gupta

    Journal of Microscopy
    |June 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hidden Variation in Microsatellite Loci: Utility and Implications for Forensic DNA Analysis.

    Forensic science review·2015
    Same author

    Identification and typing of Francisella tularensis with a highly automated genotyping assay.

    Letters in applied microbiology·2012
    Same author

    An Elliptical Crystal Spectrometer Suitable for EXAFS Studies of Laser Compressed Materials and for High Resolution X-Ray Spectroscopy.

    Journal of X-ray science and technology·2011
    Same author

    Counter for use in scattering and disintegration experiments.

    The Review of scientific instruments·2010
    Same author

    Fast-electron transport and heating of solid targets in high-intensity laser interactions measured by K alpha fluorescence.

    Physical review. E, Statistical, nonlinear, and soft matter physics·2006
    Same author

    Non-arteritic anterior ischaemic optic neuropathy and the treatment of erectile dysfunction.

    The British journal of ophthalmology·2006
    Same journal

    In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

    Journal of microscopy·2026
    Same journal

    The future of DXA: How AI is transforming bone health diagnostics.

    Journal of microscopy·2026
    Same journal

    The Origins of Ploem's Filter Cube: A Pandora's Box.

    Journal of microscopy·2026
    Same journal

    The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

    Journal of microscopy·2026
    Same journal

    Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

    Journal of microscopy·2026
    Same journal

    Modular training resources for bioimage analysis.

    Journal of microscopy·2026
    See all related articles

    Quantitative X-ray analysis of diffusible elements in cryosections faces challenges. Continuum-normalization is preferred for finer resolution, and avoiding sample evaporation before freeze-quenching is crucial for accurate results.

    Area of Science:

    • Biomedical science
    • Analytical chemistry
    • Microscopy

    Background:

    • Quantitative analysis of diffusible elements in biological samples is essential for understanding cellular processes.
    • Cryosections are commonly used for preserving cellular structures and elemental distributions.
    • X-ray spectrometry is a key technique for elemental analysis in biological tissues.

    Purpose of the Study:

    • To review challenges in quantitative analysis of diffusible elements within cryosections.
    • To compare different X-ray data processing methods for elemental quantification.
    • To highlight potential sources of error and limitations in the analysis.

    Main Methods:

    • Review of two primary methods for obtaining concentrations from X-ray data: characteristic radiation alone and continuum-normalization.

    Related Experiment Videos

  • Discussion of spatial resolution capabilities for different X-ray spectrometry techniques.
  • Consideration of sample preparation artifacts, including contamination and evaporation.

    • Main Results:

    • Both characteristic radiation and continuum-normalization methods are suitable at the cellular level.
    • Continuum-normalization is preferable for achieving finer spatial resolution.
    • Evaporation prior to freeze-quenching can significantly alter elemental concentrations in small samples.

    Conclusions:

    • Combining both X-ray analysis methods is recommended, particularly for frozen-hydrated sections without peripheral standards.
    • Careful sample handling is critical to prevent redistribution and evaporation artifacts.
    • Specific elements like chlorine and sodium present unique analytical challenges and limitations.