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Indoleamine compartmentation in human blood

E M Demet, A E Halaris, S Bhatarakamol

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |October 16, 1978
    PubMed
    Summary

    New fluorimetric methods accurately measure serotonin (5-HT), 5-HIAA, and tryptophan (TRP) in blood. These techniques precisely quantify these crucial compounds in plasma, platelets, and RBCs for enhanced research.

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    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Clinical Chemistry

    Background:

    • Accurate quantification of neurotransmitters and amino acids in blood is essential for understanding various physiological and pathological processes.
    • Existing methods for determining 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), and tryptophan (TRP) may lack precision or require separate analyses.
    • Understanding the distribution of these compounds within different blood compartments (plasma, platelets, red blood cells) is crucial for accurate interpretation of results.

    Purpose of the Study:

    • To develop and describe improved fluorimetric methods for the simultaneous determination of 5-HT, 5-HIAA, and TRP in human plasma, platelets, and red blood cells (RBCs).
    • To establish the distribution of 5-HT, 5-HIAA, and TRP across different blood cellular and fluid compartments.
    • To provide reliable analytical tools for researchers studying serotonergic pathways and amino acid metabolism.

    Main Methods:

    • Development of ion exchange chromatography-based fluorimetric assays for simultaneous quantification of 5-HT and 5-HIAA in the same blood sample.
    • Modification of an existing extraction method for the fluorimetric determination of tryptophan (TRP).
    • Application of these methods to analyze plasma, platelet, and RBC fractions from blood samples.

    Main Results:

    • All measured 5-HT was found to be localized within the platelet fraction, with a concentration of 122.6 ng/ml.
    • 5-HIAA exhibited a distribution between plasma (78.3 ng/ml) and RBC compartments (124.0 ng/ml).
    • Tryptophan was detected in all three compartments, with the highest concentrations in plasma (4.3 microgram/ml) and RBCs (1.7 microgram/ml).

    Conclusions:

    • The described fluorimetric methods offer improved accuracy and efficiency for measuring 5-HT, 5-HIAA, and TRP in distinct blood components.
    • The findings elucidate the specific distribution patterns of these key biomolecules within the blood, aiding in the interpretation of their physiological roles.
    • These validated methods are valuable tools for clinical diagnostics and biomedical research involving serotonin and tryptophan metabolism.

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