Purification and reassembly of tubulin from outer doublet microtubules

Mutation in the beta-tubulin signature motif suppresses microtubule GTPase activity and dynamics, and slows mitosis.

Biochemistry·2001

Detection of GTP and Pi in wild-type and mutated yeast microtubules: implications for the role of the GTP/GDP-Pi cap in microtubule dynamics.

Biochemistry·1998

Site-directed mutagenesis of putative GTP-binding sites of yeast beta-tubulin: evidence that alpha-, beta-, and gamma-tubulins are atypical GTPases.

Biochemistry·1995

beta-Tubulin mutation suppresses microtubule dynamics in vitro and slows mitosis in vivo.

Cell motility and the cytoskeleton·1995

Microtubule dynamics modulated by guanosine triphosphate hydrolysis activity of beta-tubulin.

Science (New York, N.Y.)·1994

Purification and biochemical characterization of tubulin from the budding yeast Saccharomyces cerevisiae.

Biochemistry·1993

Flow cytometric distinction of early apoptotic, late apoptotic and primary necrotic morphological markers in Saccharomyces cerevisiae.

Methods in cell biology·2026

CRISPR-Cas9 screen to identify genes regulating cell death.

Methods in cell biology·2026

Lipid ROS measurement by spectral flow cytometry based on the ratiometric dye BODIPY™ C11 581/591.

Methods in cell biology·2026

Isolation, characterization, and experimental applications of apoptotic bodies in vitro.

Methods in cell biology·2026

Assessment of microglial responsiveness after irradiation in mouse organotypic hippocampus or patient-derived tissue cultures.

Methods in cell biology·2026

In vitro protocols for inducing and assessing endotoxin tolerance in human and murine macrophages.

Methods in cell biology·2026

Distinct tubulin C-terminal tails control the efficiency of a microtubule severing machine.

The Journal of chemical physics·2026

Cellulose-Binding Electron-Transfer Cytochrome Composing AA8 and CBM104 from the Wood-Rotting Fungus Armillaria tabescens.

Journal of applied glycoscience·2026

Structure, morphology, and optical properties of polyacrylamide/poly (vinyl alcohol) (PAM/PVA) polymer blend containing MnO₂ nanoparticles at different concentrations.

BMC chemistry·2026

A Covalent Brush-on-Brush Polymer Programs Ordered Intracellular Activation of a Mitochondria-Disrupting Peptide.

Angewandte Chemie (International ed. in English)·2026

Regiochemistry controlled interfacial recognition of arabinoxylans on cellulose.

International journal of biological macromolecules·2026

Dynamic Polycomb-CBC crosstalk orchestrates mRNA production at transcriptionally active loci in plants.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Videos

Purification and reassembly of tubulin from outer doublet microtubules

Related Concept Videos

Mutation in the beta-tubulin signature motif suppresses microtubule GTPase activity and dynamics, and slows mitosis.

Detection of GTP and Pi in wild-type and mutated yeast microtubules: implications for the role of the GTP/GDP-Pi cap in microtubule dynamics.

Site-directed mutagenesis of putative GTP-binding sites of yeast beta-tubulin: evidence that alpha-, beta-, and gamma-tubulins are atypical GTPases.

beta-Tubulin mutation suppresses microtubule dynamics in vitro and slows mitosis in vivo.

Microtubule dynamics modulated by guanosine triphosphate hydrolysis activity of beta-tubulin.

Purification and biochemical characterization of tubulin from the budding yeast Saccharomyces cerevisiae.

Flow cytometric distinction of early apoptotic, late apoptotic and primary necrotic morphological markers in Saccharomyces cerevisiae.

CRISPR-Cas9 screen to identify genes regulating cell death.

Lipid ROS measurement by spectral flow cytometry based on the ratiometric dye BODIPY™ C11 581/591.

Isolation, characterization, and experimental applications of apoptotic bodies in vitro.

Assessment of microglial responsiveness after irradiation in mouse organotypic hippocampus or patient-derived tissue cultures.

In vitro protocols for inducing and assessing endotoxin tolerance in human and murine macrophages.

Distinct tubulin C-terminal tails control the efficiency of a microtubule severing machine.

Cellulose-Binding Electron-Transfer Cytochrome Composing AA8 and CBM104 from the Wood-Rotting Fungus Armillaria tabescens.

Structure, morphology, and optical properties of polyacrylamide/poly (vinyl alcohol) (PAM/PVA) polymer blend containing MnO₂ nanoparticles at different concentrations.

A Covalent Brush-on-Brush Polymer Programs Ordered Intracellular Activation of a Mitochondria-Disrupting Peptide.

Regiochemistry controlled interfacial recognition of arabinoxylans on cellulose.

Dynamic Polycomb-CBC crosstalk orchestrates mRNA production at transcriptionally active loci in plants.

Related Experiment Videos

Purification and reassembly of tubulin from outer doublet microtubules

Related Concept Videos

Related Articles