Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cadmium-binding proteins in human organs

M Sato, Y Takizawa

    Toxicology Letters
    |May 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Cadmium-binding proteins (CdBPs) primarily bind cadmium in human kidney and liver, mainly to metallothionein. Other organs like the pancreas and thyroid also show CdBPs, suggesting their role in cadmium accumulation and distribution.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Topography of cat medullary ventral surface hypoxic acidification.

    Journal of applied physiology (Bethesda, Md. : 1985)·1992
    Same author

    Ion channels on synaptic vesicle membranes studied by planar lipid bilayer method.

    Biophysical journal·1992
    Same author

    Nucleotide sequence of cDNA for porcine heme oxygenase and its expression in Escherichia coli.

    Biochemistry international·1992
    Same author

    Immunohistochemical evidence that rat FSH cells contain beta II-subspecies of protein kinase C.

    Endocrinologia japonica·1992
    Same author

    Postnatal ontogenesis of neurons containing GABAA alpha 1 subunit mRNA in the rat forebrain.

    Brain research. Molecular brain research·1992
    Same author

    Immunocytochemical and in situ hybridization evidence for the coexistence of GABA and tyrosine hydroxylase in the rat locus ceruleus.

    The Anatomical record·1992

    Area of Science:

    • Toxicology
    • Biochemistry
    • Human Health

    Background:

    • Cadmium (Cd) is a toxic heavy metal with significant health implications.
    • Understanding cadmium-binding proteins (CdBPs) is crucial for elucidating its toxicokinetics and distribution.
    • Metallothionein is a known cadmium-binding protein, but other CdBPs may exist.

    Purpose of the Study:

    • To identify and characterize cadmium-binding proteins (CdBPs) in various human organs following oral cadmium exposure.
    • To investigate the distribution and binding patterns of cadmium in different human tissues.
    • To explore the potential role of CdBPs in cadmium accumulation and toxicity.

    Main Methods:

    • Cytosol fractions from multiple human organs were analyzed using gel chromatography.

    Related Experiment Videos

  • Cadmium levels and protein binding were assessed in the separated fractions.
  • Comparison of CdBPs across different organs and species.

    • Main Results:

    • In human kidney and liver cytosols, 76-87% of cadmium was bound to metallothionein, which also contained zinc.
    • A metallothionein-like CdBP was found in pancreas cytosol, but at lower concentrations.
    • A distinct CdBP, separate from metallothionein, was identified in the thyroid gland.
    • No CdBPs were detected in organs such as the heart, muscle, or aorta.

    Conclusions:

    • Cadmium-binding proteins, particularly metallothionein, play a significant role in cadmium accumulation and distribution in human organs.
    • The kidney, liver, pancreas, and thyroid gland are key organs involved in cadmium binding and potential accumulation.
    • The presence of unique CdBPs in the thyroid suggests organ-specific mechanisms for cadmium handling.