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Updated: Aug 14, 2026

12:48
Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis
Published on: March 25, 2014
[A non-damaging procedure for DNA isolation from insect cell nuclei]
Abstract:
A gentle procedure is described for DNA isolation from Galleria mellonella cell nuclei. Nuclei lysis was performed by SDS in final concentration of 3% which was followed by DNA extraction from chromatin by a stepwise rise of NaCl concentration up to 2.5 M. After removal of nuclei insoluble components by centrifugation the native DNA was purified using the hydroxyapatite column. The method proposed provides a higher yield of the native DNA as compared with the phenol-detergent method.
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