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Updated: Aug 14, 2026

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
Published on: January 22, 2018
[Measurement of alanine aminotransferase and aspartate aminotransferase activity with a 2-channel automatic flow
Abstract:
A flow automaton for the estimation of the enzyme activities of the ALAT and ASAT is described: 1. In precision and exactness the automaton achieves results comparable to the manual UV-test. The VK are clearly below 5%. 2. Sera with enzyme activities greater than 200 U/l are diluted with human albumin solution (20 g/l) 1 : 5 or 1 : 10. 3. The speed of the analysis with simultaneous estimation of the activity of the ALAT and ASAT from one test is 40 tests/h. 4. The technological disadvantages of the colour test regarding the kinetic measurement are vastly evaded by the selected device parameters and conditions.
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