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Related Experiment Videos

Ion repulsion within membranes

R Y Tsien, S B Hladky

    Biophysical Journal
    |July 1, 1982
    PubMed
    Summary

    Electrostatic repulsion explains hydrophobic ion adsorption saturation on lipid membranes. A discrete, mobile charge model accurately fits adsorption data, outperforming previous models.

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    Area of Science:

    • Biophysics
    • Physical Chemistry
    • Membrane Science

    Background:

    • Hydrophobic ions, like tetraphenylborate, adsorb to lipid membranes.
    • Adsorption of these ions saturates at approximately 0.1 ion/(nm)2.
    • Previous models (smeared charge, hexagonal lattice) inadequately explain this saturation.

    Purpose of the Study:

    • To quantitatively explain the saturation of hydrophobic ion adsorption on lipid membranes.
    • To develop and validate a new physical model for ion adsorption.
    • To improve the fit to experimental adsorption data.

    Main Methods:

    • Modeling ion adsorption as discrete, mobile particles within the lipid membrane.
    • Utilizing electrostatic repulsion to explain adsorption saturation.
    • Employing a virial expansion of the electrochemical potential as a function of surface density.
    • Calculating the first few terms of the virial expansion and approximating higher-order terms.

    Main Results:

    • The discrete, mobile charge model quantitatively explains adsorption saturation.
    • The model accurately fits experimental adsorption data for tetraphenylborate up to 0.1 ion/(nm)2.
    • The model demonstrates superior performance compared to smeared charge and hexagonal lattice models.
    • The model incorporates two adjustable parameters: adsorption site depth and intrinsic adsorption constant.

    Conclusions:

    • Electrostatic repulsion between discrete, mobile ions is the key mechanism for adsorption saturation.
    • The developed mobile, discrete charge model provides a more accurate description of ion-membrane interactions.
    • This model offers improved quantitative fits to experimental equilibrium adsorption data.

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