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Related Experiment Videos

A model system for studying metastasis using the embryonic chick

A F Chambers, R Shafir, V Ling

    Cancer Research
    |October 1, 1982
    PubMed
    Summary
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    A new assay uses ouabain sensitivity to detect viable rodent cells in chick embryos, aiding metastasis research. This method improves the chick embryo model by overcoming time limitations and detecting cells before tumors are visible.

    Area of Science:

    • Developmental biology
    • Cancer research
    • Cell biology

    Background:

    • The chick embryo is a valuable model for studying cancer metastasis.
    • Previous limitations in the chick embryo model include time constraints and difficulty in detecting early-stage metastasis.
    • A method to quantify viable metastatic cells is needed to enhance the model's utility.

    Purpose of the Study:

    • To describe a novel assay for recovering viable individual rodent cells from chick embryo organs.
    • To evaluate the chick embryo as a model system for metastasis research using this assay.
    • To characterize cell line metastatic potential and compare it with the novel assay's results.

    Main Methods:

    • Development of an assay based on differential sensitivity of chick and rodent cells to ouabain.

    Related Experiment Videos

  • Characterization of cell lines for tumor formation on the chorioallantoic membrane.
  • Assessment of metastasis formation after injection into chorioallantoic membrane veins.
  • Comparison of experimental metastasis results with the ouabain-plating assay.

    • Main Results:

    • The ouabain-plating assay successfully recovered viable individual rodent cells from various chick embryo organs.
    • The assay detected viable metastatic cells even when tumors were not yet macroscopically or microscopically detectable.
    • Results from the assay correlated with traditional methods of assessing metastatic potential.

    Conclusions:

    • The described assay enables the detection of viable metastatic cells in the chick embryo model.
    • This method overcomes previous time limitations, enhancing the chick embryo's utility for metastasis studies.
    • The assay facilitates the study of metastasis at an earlier, sub-clinical stage.