Application of high-powered microwave irradiation for acetylcholine analysis in mouse brain

Acetylcholine (ACh) levels in whole brains or discrete areas of mice brains were measured after sacrificing by decapitation, conventional or high-powered microwave irradiation. ACh levels in mice brains varied according to the time required to inactivate the enzymes. ACh levels after high-powered microwave irradiation for a shorter time were significantly higher than those after conventional irradiation.