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A new in vivo method for quantitatively evaluating local anesthetics

F M Lieeman, D Brown, P Friedman

European Journal of Pharmacology

|October 15, 1978

Summary

A novel hamster cheek pouch model quantifies local anesthetic blockade. Ketocaine showed a slow onset, making it impractical for topical use despite its anesthetic potential.

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Area of Science:

Pharmacology
Neuroscience
Anesthesiology

Background:

Evaluating local anesthetic efficacy requires reliable methods to assess nerve blockade.
The hamster cheek pouch offers a unique anatomical structure for topical drug absorption and nerve studies.

Purpose of the Study:

To develop and validate a quantitative method for assessing local anesthetic onset and duration using hamster cheek pouch reflex.
To evaluate the topical anesthetic efficacy of Ketocaine using this novel model.

Main Methods:

Utilized reflex contraction of the hamster cheek pouch retractor muscle to measure blockade.
Quantified reflex tension as a function of afferent nerve conductivity in the pouch.
Validated the model against existing animal models and clinical trial data.

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Main Results:

The developed method provides a quantitative measure of local anesthetic blockade.
Ketocaine demonstrated a long latency of onset for blockade when administered topically.
The model successfully differentiated anesthetic properties.

Conclusions:

The hamster cheek pouch retractor muscle reflex is a viable model for evaluating local anesthetic properties.
Ketocaine's current formulation is impractical for clinical topical anesthesia due to its slow onset.