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Human leukocyte inhibitory factor (LIF): a lymphocyte mediator with esteratic properties
Federation Proceedings
|November 1, 1978
Summary
Lymphocytes release leukocyte inhibitory factor (LIF), a protein, that stops polymorphonuclear (PMN) leukocyte migration. LIF also enhances PMN functions and can induce neutrophil immobilization factor release.
Area of Science:
- Immunology
- Cell Biology
Background:
- Lymphocytes produce soluble mediators that influence other immune cells.
- Leukocyte inhibitory factor (LIF) is a known lymphocyte mediator affecting polymorphonuclear (PMN) leukocytes.
Purpose of the Study:
- To characterize the properties and functions of leukocyte inhibitory factor (LIF).
- To investigate the mechanism by which LIF affects polymorphonuclear (PMN) leukocyte migration.
Main Methods:
- Stimulation of human or guinea pig lymphocytes with antigen or mitogens.
- Biochemical characterization of LIF, including its esterase activity.
- Assays to evaluate LIF's effect on PMN leukocyte functions and migration.
Main Results:
- LIF selectively inhibits random PMN leukocyte migration.
- LIF exhibits esterase properties and can utilize synthetic substrates.
- LIF enhances PMN adherence, phagocytosis, metabolism, and electrophoretic mobility.
- LIF induces the release of neutrophil immobilization factor from PMNs, amplifying its inhibitory effect.
Conclusions:
- LIF is a unique protein mediator with esterase activity that regulates PMN migration.
- LIF enhances PMN functions and modulates their movement through induced factors.
- Biochemical assays for LIF detection may be feasible using esterase inhibitors.