Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new chamber for rapid sperm count and motility estimation

A Makler

    Fertility and Sterility
    |September 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A new objective method for scoring human sperm hyperactivation based on head axis angle deviation.

    International journal of andrology·2001
    Same author

    Potential sources of error with the Makler counting chamber.

    Fertility and sterility·2000
    Same author

    Evaluation of the SQA IIB: a new version of a sperm quality analyzer.

    Fertility and sterility·1999
    Same author

    Dynamic aspects concerned with the mechanism of separating motile sperm from nonmotile sperm, leukocytes, and debris with the use of high-density Percoll gradients.

    Fertility and sterility·1998
    Same author

    Standardization of counting chambers for sperm analysis.

    Fertility and sterility·1997
    Same author

    Clinical, biochemical, and genetic findings in a large pedigree of male and female patients with 5 alpha-reductase 2 deficiency.

    The Journal of clinical endocrinology and metabolism·1996

    A novel 10-micron deep chamber facilitates accurate sperm count and motility analysis in undiluted samples. This tool enhances precision and reliability for sperm analysis, offering a valuable improvement for fertility assessments.

    Area of Science:

    • Reproductive biology
    • Andrology
    • Medical device technology

    Background:

    • Accurate sperm analysis is crucial for male fertility assessment.
    • Existing methods for sperm count and motility can be time-consuming and require sample dilution.
    • A need exists for improved tools for rapid and precise semen evaluation.

    Purpose of the Study:

    • To describe a new 10-micron deep chamber for sperm count and motility estimation.
    • To evaluate the precision, accuracy, and reliability of this new chamber compared to established methods.
    • To assess the utility of the chamber for analyzing undiluted semen samples.

    Main Methods:

    • A novel 10-micron deep chamber was designed for sperm analysis.
    • Sperm counts were performed directly from undiluted, preheated samples using an eyepiece grid.

    Related Experiment Videos

  • Motility was assessed using the chamber, allowing for comparison across different samples and times.

    • Main Results:

    • The chamber enables free horizontal movement of spermatozoa in a single focal plane.
    • Sperm counts demonstrated high precision, accuracy, and reliability compared to the hemocytometric method.
    • Motility estimation yielded superior results compared to the ordinary slide technique.

    Conclusions:

    • The new chamber provides a precise and reliable method for sperm count and motility assessment.
    • Its ability to analyze undiluted samples simplifies the process and improves accuracy.
    • The chamber is a valuable tool for improving the efficiency and quality of sperm analysis in clinical settings.