Characterization of the forms of bovine liver adenosine deaminase

1. The A and C forms of bovine liver adenosine deaminase (adenosine aminohydrolase; EC 3.5,4.4) have been separated. 2. The proportion of two forms is dependent on ionic strength of solution. 3. By gel filtration, in presence of 6 M urea, and A form is dissociated into the C form and the binding factor and both are also separated. By removal of urea the A form is again obtained. 4. The molecular weights of two forms and binding factor, kinetic parameters have been determined.