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Qa-1 and Qa-2 expression on CFU-s

M A Lynes, S Tonkonogy, L Flaherty

    Journal of Immunology (Baltimore, Md. : 1950)
    |September 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Multipotential stem cells, known as colony-forming unit-spleen (CFU-s), express both Qa-1 and Qa-2 antigens. These findings highlight the broad presence of Qa antigens within the hematopoietic system.

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    Area of Science:

    • Immunogenetics
    • Hematopoietic Stem Cell Biology

    Background:

    • Multipotential stem cells are crucial for hematopoiesis.
    • Colony-forming unit-spleen (CFU-s) are a functional assay for detecting these stem cells.
    • Qa antigens are a group of cell surface molecules with roles in immune regulation.

    Purpose of the Study:

    • To determine the expression of Qa-1 and Qa-2 antigens on CFU-s.
    • To investigate the distribution of Qa antigens within the hematopoietic system.

    Main Methods:

    • Bone marrow cells were injected into irradiated syngeneic hosts to form splenic colonies (CFU-s).
    • Bone marrow cells were pretreated with specific anti-Qa antisera or monoclonal antibodies in the presence of complement.
    • The reduction in CFU-s formation was measured to infer antigen expression on the stem cells.

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    Main Results:

    • Pretreatment with anti-Qa-1.2 and anti-Qa-1.1 sera significantly reduced CFU-s, indicating expression of both Qa-1.1 and Qa-1.2 on CFU-s.
    • Pretreatment with anti-Qa-2,3 sera and a monoclonal anti-Qa-2 antibody also significantly reduced CFU-s, demonstrating Qa-2 expression on CFU-s.
    • These results were consistent across different mouse strains.

    Conclusions:

    • Both Qa-1 and Qa-2 antigens are expressed on multipotential hematopoietic stem cells (CFU-s).
    • Qa antigens show a wide distribution throughout the hematopoietic system.
    • This study contributes to understanding the role of Qa antigens in hematopoiesis.