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Related Experiment Videos

Size regulation in Dictyostelium

W Kopachik

    Journal of Embryology and Experimental Morphology
    |April 1, 1982
    PubMed
    Summary

    Dictyostelium discoideum slug size is determined by tip inhibition and cell resistance. Larger slugs form when cells are easily inhibited, smaller slugs when cells resist inhibition.

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    Area of Science:

    • Cellular biology
    • Developmental biology
    • Microbiology

    Background:

    • Dictyostelium discoideum aggregates form slugs.
    • Slug size regulation is a key developmental process.
    • Understanding factors influencing slug size is crucial.

    Purpose of the Study:

    • To investigate the factors determining Dictyostelium discoideum slug size.
    • To test the hypothesis that slug size is controlled by tip inhibition and cell resistance.
    • To quantify the parameters of tip inhibition.

    Main Methods:

    • Examined two Dictyostelium discoideum strains differing in size.
    • Developed an assay for tip inhibition.
    • Measured inhibition volume, distance, and duration.
    • Assessed the effect of tip size and cell mass shape on size regulation.

    Main Results:

    • Slug size is determined by tip's inhibition ability and surrounding cells' resistance.
    • Easily inhibited cells produce large slugs; resistant cells produce small slugs.
    • Inhibition spreads via simple diffusion from the tip.
    • Smaller strain exhibited greater resistance to inhibition.

    Conclusions:

    • Tip inhibition and cell resistance are critical for Dictyostelium discoideum slug size.
    • Inhibitor diffusion from the tip regulates slug size.
    • Strain-specific differences in resistance contribute to size variation.

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