Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An efficient method for loading indium-111 into liposomes using acetylacetone

P L Beaumier, K J Hwang

    Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
    |September 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Advanced Methods for Analyzing in-Situ Observations of Magnetic Reconnection.

    Space science reviews·2024
    Same author

    Electron-scale dynamics of the diffusion region during symmetric magnetic reconnection in space.

    Science (New York, N.Y.)·2018
    Same author

    Synthesis and Antifungal Activities of Furoxan Derivatives Designed as Novel Fungicide.

    Bioscience, biotechnology, and biochemistry·2016
    Same author

    Post-stroke restless leg syndrome and periodic limb movements in sleep.

    Acta neurologica Scandinavica·2016
    Same author

    Intracranial stent placement for recanalization of acute cerebrovascular occlusion in 32 patients.

    AJNR. American journal of neuroradiology·2010
    Same author

    Inhibition of Orientia tsutsugamushi infection by a truncated recombinant 56-kDa outer membrane protein.

    Letters in applied microbiology·2010
    Same journal

    Overall Survival with [<sup>177</sup>Lu]Lu-PSMA-617 Versus [<sup>177</sup>Lu]Lu-PSMA I&T: A Propensity Score-Matched Real-World Analysis.

    Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
    Same journal

    Toward a Biopsy-Free Diagnosis of Prostate Cancer: Potential of Combined <sup>18</sup>F-Flotufolastat PSMA PET and mpMRI.

    Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
    Same journal

    PSMA PET/CT-Targeted Biopsy in Men with Negative or Equivocal Multiparametric MRI and Exploratory Dynamic Total-Body PET: The FUPERMAN Study.

    Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
    Same journal

    Erratum.

    Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
    Same journal

    Live from 2026 SNMMI Annual Meeting in Los Angeles!

    Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
    Same journal

    CAR T-Cell Therapy for Cancer: Updates and Challenges for Response Assessment.

    Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
    See all related articles

    Acetylacetone efficiently loads Indium-111 (In-111) into liposomes by mediating ion transport. This method successfully concentrates In-111 within liposomes, which remain stable in serum.

    Area of Science:

    • Nanotechnology
    • Radiochemistry
    • Biomedical Engineering

    Background:

    • Liposomes are widely used drug delivery systems.
    • Efficiently encapsulating radioisotopes like Indium-111 (In-111) into liposomes is crucial for imaging and therapy.
    • Current methods face challenges in achieving high loading efficiency and stability.

    Purpose of the Study:

    • To develop an efficient method for loading high levels of In-111 into liposomes.
    • To investigate the mechanism of In-111 transport and retention within liposomes.
    • To assess the stability of In-111 loaded liposomes in biological conditions.

    Main Methods:

    • Utilized acetylacetone, a water-soluble lipophilic chelate, to form an acetylacetone-In-111 complex.
    • Incubated the complex with liposomes pre-loaded with nitrilotriacetic acid (NTA).

    Related Experiment Videos

  • Conducted loading in isotonic saline with Tris-HCl buffer at room temperature.
  • Evaluated liposome stability by incubating loaded liposomes in serum at 37°C for 24 hours.

    • Main Results:

    • Achieved efficient loading of high In-111 levels into the inner aqueous compartment of liposomes.
    • Acetylacetone mediated the transport of In-111 across the lipid bilayer to the encapsulated NTA.
    • Up to 90% of acetylacetone-chelated In-111 was internalized and bound by NTA.
    • Loaded liposomes demonstrated excellent retention of In-111 when incubated with serum.

    Conclusions:

    • Acetylacetone is an effective agent for the efficient loading of In-111 into liposomes.
    • The developed method ensures high encapsulation efficiency and stability of In-111 within liposomes.
    • These findings support the potential of In-111 loaded liposomes for biomedical applications.