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Microdissection by ultrasonication after prolonged OsO4 fixation: a technique for scanning electron microscopy
Journal of Submicroscopic Cytology
|January 1, 1982
Summary
A novel electron microscopy technique selectively removes mammalian tissue epithelial cells, preserving basal laminas for enhanced visualization of microcirculation and histological structures.
Area of Science:
- Electron Microscopy
- Histology
- Cell Biology
Background:
- Standard electron microscopy techniques can obscure fine details of tissue architecture.
- Visualizing microcirculation and basal laminas often requires specialized preparation methods.
Purpose of the Study:
- To introduce a new tissue preparation method for electron microscopy.
- To enable selective dissection and enhanced visualization of mammalian tissues, particularly microcirculation and basal laminas.
Main Methods:
- Tissue preparation involves prolonged osmication for brittlization.
- Dehydration using pure acetone followed by ultrasonication.
- Selective removal of epithelial cells while preserving collagen-rich structures like basal laminas.
Main Results:
- The technique effectively fragments and removes epithelial cells.
- Epithelial and vascular basal laminas demonstrate resistance to ultrasonication.
- The degree of removal is dependent on tissue type and ultrasonic treatment duration.
Conclusions:
- This method enhances the visualization of microcirculation and basal laminas.
- It offers a valuable tool for studying histological relationships.
- Potential for discovering unrecognized tissue structures and interactions.