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Pyosemia and carriage of chlamydia and ureaplasma in infertile men
Abstract:
Chlamydia trachomatis and Ureaplasma urealyticum have been implicated as causative organisms in infections involving the male and female urogenital tracts. Seminal fluid, anterior urethral swabs and first-voided urine specimens from men undergoing infertility evaluation, with and without pyosemia, or anterior urethritis were cultured for Chlamydia trachomatis and Ureaplasma urealyticum. The method used to isolate Chlamydia trachomatis involved cytochalasin-B treated McCoy cells, and NYC and A7 solid media were used for the isolation of Ureaplasma urealyticum. Isolation of Chlamydia trachomatis from seminal fluid has not been possible even in the face of pyosemia and the presence of Chlamydia trachomatis in urine and urethral swab material. The reasons for the inability to culture Chlamydia trachomatis will be explored.
Insights
Chlamydia trachomatis and Ureaplasma urealyticum are linked to urogenital infections. Researchers found Chlamydia trachomatis could not be cultured from seminal fluid, despite detection in urine and swabs.
Area of Science:
- Microbiology
- Urology
- Infectious Diseases
Background:
- Chlamydia trachomatis and Ureaplasma urealyticum are recognized pathogens in urogenital tract infections.
- These infections can impact male and female reproductive health, including infertility.
Purpose of the Study:
- To investigate the presence of Chlamydia trachomatis and Ureaplasma urealyticum in male urogenital samples.
- To evaluate the feasibility of culturing Chlamydia trachomatis from seminal fluid.
Main Methods:
- Specimens included seminal fluid, anterior urethral swabs, and first-voided urine from men undergoing infertility evaluation.
- Chlamydia trachomatis isolation utilized cytochalasin-B treated McCoy cells.
- Ureaplasma urealyticum isolation employed NYC and A7 solid media.
Main Results:
- Chlamydia trachomatis was detected in urine and urethral swab specimens.
- Despite pyosemia and presence in other samples, Chlamydia trachomatis could not be isolated from seminal fluid.
- Ureaplasma urealyticum isolation methods were employed.
Conclusions:
- Current methods are insufficient for culturing Chlamydia trachomatis from seminal fluid.
- Further research is needed to understand the reasons for this isolation failure and its clinical implications.