Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse spot tests with alkylnitrosoureas

T Shibuya, T Murota, K Tutikawa

    Mutation Research
    |May 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Alkylnitrosoureas like ENU, PNU, and BNU induced color spots in mice, suggesting mutagenic effects. N-methyl-N-nitrosourea (MNU) caused teratogenicity but no spots, while reduced testes weight was observed in offspring.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Reply by Authors.

    The Journal of urology·2019
    Same author

    Laparoscopic adrenalectomy for pheochromocytoma: a literature review.

    Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2002
    Same author

    [Transperitoneal laparoscopic radical prostatectomy with the Montsouris technique: experience in initial 5 cases].

    Nihon Hinyokika Gakkai zasshi. The japanese journal of urology·2001
    Same author

    Transperitoneal anterior laparoscopic adrenalectomy: the easiest technique.

    Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2000
    Same author

    [Efficacy of single-dose therapy with levofloxacin for acute cystitis: comparison to three-day therapy].

    Hinyokika kiyo. Acta urologica Japonica·2000
    Same author

    Deletion of the carboxyl-terminal exons of K-sam/FGFR2 by short homology-mediated recombination, generating preferential expression of specific messenger RNAs.

    Cancer research·2000

    Area of Science:

    • Toxicology
    • Genetics
    • Mammalian models

    Background:

    • Alkylnitrosoureas are a class of chemical mutagens.
    • Assessing the mutagenic potential of these compounds is crucial for understanding their health risks.

    Purpose of the Study:

    • To evaluate the mutagenic effects of four alkylnitrosoureas (MNU, ENU, PNU, BNU) in a mouse model.
    • To investigate the dose-dependency of the observed mutagenic effects.

    Main Methods:

    • Spot tests were performed on male and female C57BL/6 mice (PW strain).
    • Mice were exposed to varying doses of N-methyl-N-nitrosourea (MNU), N-ethyl-N-nitrosourea (ENU), N-propyl-N-nitrosourea (PNU), and N-butyl-N-nitrosourea (BNU).
    • Testes weight in F1 male offspring was measured.

    Related Experiment Videos

    Main Results:

    • ENU, PNU, and BNU induced observable color spots, with frequency correlating to dose.
    • MNU exhibited severe teratogenic effects but did not produce color spots.
    • A significant reduction in testes weight was noted in F1 male mice from the treated groups.

    Conclusions:

    • ENU, PNU, and BNU demonstrate mutagenic potential in mice.
    • The observed effects suggest potential mutagenicity in both somatic and germ cells.
    • Further research is warranted to elucidate the mechanisms of alkylnitrosourea-induced mutations.