Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The basic repeat unit of a Chironomus Balbiani ring gene

H Bäumlein, U Wobus, S A Gerbi

    Nucleic Acids Research
    |July 10, 1982
    PubMed
    Summary

    Researchers analyzed the Balbiani ring b (BRb) gene in Chironomus thummi. They discovered extensive tandem repeats within the gene, forming a complex internal organization with shorter, repeating peptide sequences.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Parthenogenetic egg cells of wheat: cellular and molecular studies.

    Sexual plant reproduction·2014
    Same author

    Abundant embryonic mRNA in field bean (Vicia faba L.) codes for a new class of seed proteins: cDNA cloning and characterization of the primary translation product.

    Plant molecular biology·2013
    Same author

    Long-term cultures of barley synthesize and correctly deposit seed storage proteins.

    Plant cell reports·2013
    Same author

    Transient expression of a lysine-rich vicilin gene ofVicia faba in barley endosperm detected by immunological tissue printing after particle bombardment.

    Plant cell reports·2013
    Same author

    The spalt-related gene of Drosophila melanogaster is a member of an ancient gene family, defined by the adjacent, region-specific homeotic gene spalt.

    Development genes and evolution·2013
    Same author

    Widespread divergence between incipient Anopheles gambiae species revealed by whole genome sequences.

    Science (New York, N.Y.)·2010

    Area of Science:

    • Molecular Biology
    • Genetics
    • Developmental Biology

    Background:

    • The Balbiani ring b (BRb) gene in Chironomus thummi is crucial for gene regulation.
    • Understanding the internal organization of BRb gene is key to deciphering its function.

    Purpose of the Study:

    • To investigate the internal structural organization of the Balbiani ring b (BRb) gene.
    • To characterize the repetitive elements within the BRb gene sequence.

    Main Methods:

    • Gene cloning techniques were employed to isolate the BRb gene.
    • Sequence analysis was performed to identify repetitive units and subsequences.

    Main Results:

    • The BRb gene contains approximately 80 tandemly organized repeat units (ca. 300 bp each).
    • A significant portion (242 bp) of the repeat unit shows extensive internal repetitive substructure.
    • Approximately 60% of the unit comprises short, tandemly organized subsequences encoding peptides (8-11 residues).
    • These subsequences are further composed of shorter internal repeats, forming a tripeptide consensus (Proline-Serine-Lysine).

    Conclusions:

    • The Balbiani ring b gene exhibits a highly repetitive internal organization.
    • This repetitive structure suggests specialized roles in gene regulation or protein function within Chironomus thummi.

    Related Experiment Videos