Dimethyl sulfoxide. Biomechanical effects on tendons

Area of Science:

• Biomedical Engineering
• Materials Science
• Orthopedics

Background:

• Dimethyl sulfoxide (DMSO) is a common topical agent with potential effects on connective tissues.
• Understanding DMSO's impact on tendon biomechanics is crucial for clinical applications and patient safety.

Purpose of the Study:

• To investigate the effect of topical dimethyl sulfoxide (DMSO) application on the tensile strength of Achilles tendons in mice.
• To determine the time-dependent changes in tendon mechanical properties following DMSO treatment.

Main Methods:

• Forty-eight mice underwent daily topical application of 70% DMSO solution to their Achilles tendons for up to 28 days.
• Achilles tendons were harvested and subjected to separation force analysis to measure tensile strength.
• Statistical analysis was performed to identify significant trends and components (linear, quadratic, cubic) in the force-time data.

Main Results:

• Mean separation force of Achilles tendons was significantly influenced by the duration of DMSO treatment (P = 0.0294).
• A complex pattern was observed: decreased force from 0-7 days, increased force from 7-22 days, and decreased force from 22-28 days.
• The maximum reduction in mean separation force was 20.2% at 7 days of treatment.

Conclusions:

• Topical DMSO application alters Achilles tendon mechanical properties in a time-dependent manner.
• The observed decreases in tendon separation force suggest potential vulnerability during specific treatment periods.
• Recommendations include avoiding strenuous activity during DMSO therapy to mitigate risks associated with reduced tendon strength.