Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in hepatic cytochrome P-450 by cobalt. Evidence for a role of cobalt protoporphyrin

J F Sinclair, P R Sinclair, J F Healey

    The Biochemical Journal
    |April 15, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A 5.8-kDa component of manuka honey stimulates immune cells via TLR4.

    Journal of leukocyte biology·2007
    Same author

    Development of temporal response properties and contrast sensitivity of V1 and V2 neurons in macaque monkeys.

    Journal of neurophysiology·2007
    Same author

    Method for assessing the chronic toxicity of marine and estuarine sediment-associated contaminants using the amphipod Corophium volutator.

    Marine environmental research·2007
    Same author

    Effects of perceptual learning on local stereopsis and neuronal responses of V1 and V2 in prism-reared monkeys.

    Journal of neurophysiology·2007
    Same author

    Cortical effects of brief daily periods of unrestricted vision during early monocular form deprivation.

    Journal of neurophysiology·2006
    Same author

    Osteocyte apoptosis and osteoclast presence in chicken radii 0-4 days following osteotomy.

    Calcified tissue international·2005

    Cobalt chloride (CoCl2) exposure in chick hepatocytes and rats leads to cobalt protoporphyrin formation. This process reduces haem and cytochrome P-450 levels, impacting liver function.

    Area of Science:

    • Biochemistry
    • Toxicology
    • Cell Biology

    Background:

    • Cytochrome P-450 is crucial for drug metabolism and detoxification.
    • Heme synthesis is essential for cytochrome P-450 function.
    • Metal exposure can disrupt cellular processes.

    Purpose of the Study:

    • To investigate the effect of cobalt chloride on heme and cytochrome P-450 synthesis.
    • To determine the mechanism of cobalt-induced porphyrin formation.
    • To analyze the association of cobalt protoporphyrin with hepatic proteins.

    Main Methods:

    • Exposure of cultured chick-embryo hepatocytes to cobalt chloride and allylisopropylacetamide.
    • Treatment of rats with cobalt chloride and 5-aminolaevulinate.
    • Analysis of hepatic microsomal fractions using polyacrylamide gel electrophoresis.

    Related Experiment Videos

    Main Results:

    • Cobalt chloride exposure led to cobalt protoporphyrin formation in chick hepatocytes and rat liver.
    • A reciprocal decrease in heme and cytochrome P-450 was observed.
    • Cobalt protoporphyrin was found to associate with proteins of 50000-53000 mol.wt.

    Conclusions:

    • Cobalt protoporphyrin formation occurs at the expense of heme synthesis, reducing cytochrome P-450 levels.
    • The formed cobalt protoporphyrin may be incorporated into apocytochrome P-450.
    • Cobalt exposure disrupts heme metabolism and cytochrome P-450 homeostasis.