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Related Experiment Videos

Aggregation of human platelets by amniotic fluid

H H Salem, W A Walters, J L Perkin

    British Journal of Obstetrics and Gynaecology
    |September 1, 1982
    PubMed
    Summary

    Amniotic fluid contains collagen and other substances that cause irreversible platelet aggregation and shorten plasma clotting time. Management of pregnancy coagulopathy may require combined anticoagulant and antiplatelet therapies.

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    Area of Science:

    • Biochemistry
    • Hematology
    • Obstetrics

    Background:

    • Amniotic fluid plays a crucial role in fetal development and maternal health.
    • Understanding the hemostatic properties of amniotic fluid is essential for managing obstetric complications.

    Purpose of the Study:

    • To investigate the procoagulant and platelet-aggregating activities of human amniotic fluid.
    • To identify the components within amniotic fluid responsible for these activities.

    Main Methods:

    • Analysis of 24 amniotic fluid samples for effects on human platelets and plasma recalcification time.
    • High-speed ultracentrifugation to isolate active material.
    • Collagenase inhibition assays, polyacrylamide-gel electrophoresis, and hydroxyproline assays to confirm collagen presence.

    Main Results:

    • All amniotic fluid samples induced irreversible platelet aggregation.
    • Active material precipitated upon ultracentrifugation and was inhibited by collagenase.
    • Amniotic fluid significantly shortened plasma recalcification time, indicating procoagulant activity.
    • Collagen, thromboplastin, and other unidentified factors contribute to amniotic fluid's clot-promoting effects.

    Conclusions:

    • Amniotic fluid contains potent activators of platelets and clotting factors.
    • These findings support the presence of clot-promoting substances in amniotic fluid.
    • Combined anticoagulant and antiplatelet drug therapy may be optimal for managing pregnancy-related coagulopathy.

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