Related Experiment Video

Updated: Aug 16, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Raman spectroscopic study of the interaction between sulfate anion and an imidazolium ring in ribonuclease A

I Harada, T Takamatsu, M Tasumi

    Biochemistry
    |July 20, 1982
    PubMed

    More Related Videos

    Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy11:13

    Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

    Published on: August 20, 2018

    Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA08:17

    Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

    Published on: July 9, 2021

    Related Concept Videos

    Riboswitches01:56

    Riboswitches

    Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
    The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
    Ribozymes02:47

    Ribozymes

    The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
    Ribozymes can be...
    Basicity of Heterocyclic Aromatic Amines01:25

    Basicity of Heterocyclic Aromatic Amines

    Heterocyclic amines, where the N atom is a part of an alicyclic system, are similar in basicity to alkylamines. Interestingly, the heterocyclic amine having a nitrogen atom as part of an aromatic ring has much less basicity than its corresponding alicyclic counterpart. For this reason, as presented in Figure 1, piperidine (pKb = 2.8) is significantly more basic than pyridine (pKb = 8.8).
    Amines to Sulfonamides: The Hinsberg Test01:23

    Amines to Sulfonamides: The Hinsberg Test

    The Hinsberg test is a method to identify primary, secondary and tertiary amines, named after its pioneer, Oscar Hinsberg. Here, amines are treated with benzenesulfonyl chloride, also known as the Hinsberg reagent, in the presence of an excess of aqueous base, followed by acidification. Based on the nature of the amines, different changes are observed.
    Generally, a primary amine reacts with the Hinsberg reagent to produce an N-substituted benzenesulfonamide. The electron-withdrawing sulfonyl...
    Aldehydes and Ketones with Amines: Imine Formation Mechanism01:23

    Aldehydes and Ketones with Amines: Imine Formation Mechanism

    Imine formation involves the addition of carbonyl compounds to a primary amine. It begins with the generation of carbinolamine through a series of steps involving an initial nucleophilic attack and then several proton transfer reactions. The second part includes the elimination of water, as a leaving group, to give the imine.
    Imines are formed under mildly acidic conditions. A pH of 4.5 is ideal for the reaction.
    If the pH is low or the solution is too acidic, the reaction slows down in the...