Scanning electron microscopy of the metal-tissue interface. I. Fixation methods and interpretation of results

Scanning electron microscopy has been used to study the interface between implants of pure metals and the muscular tissue of rats. Several problems arise in the interpretation of the observations made, due to the potential for disruption to the tissue and the exacerbation of any corrosion that may have occurred in vivo during the fixation periods. Details are given of the control experiments performed in order to ascertain the nature of potential artefacts and of the techniques eventually determined to be optimal for these purposes.