[Antiradical activity and sulfhydryl group concentration in normal and tumor tissue]

In cytosol of normal and tumor tissues obtained from rats and hamsters, antiradical activity (ARA) of bioantioxidants was assayed by the method of Glavind, while the content of sulfhydryl groups by the method of Ellman. ARA of normal tissues (liver, kidneys, stomach, large intestine, femoral muscles) was lower than the content of free SH-groups. The difference between the magnitudes under consideration was always negative in all the test tissues. In tumors of the liver, stomach, kidneys, large intestine and femoral muscle induced in rats and hamsters by chemical carcinogens, there was an increase in ARA from 21 to 125.8% as compared with respective normal tissues. The difference between ARA and the content of SH-groups in the tumors was always positive. It might be assumed that in normal tissues, ARA is primarily determined by sulfhydryl compounds. Meanwhile, apart from SH-containing compounds, the other substances also take part in the control of ARA.