New antibiotics, enaminomycins A, B and C. I. Producing organism, fermentation and isolation

New antibiotics, enaminomycins A, B and C, were found in the culture broth of streptomycete strain No. 13120, which was identified as Streptomyces baarnensis and designated as S. baarnensis No. 13120. Fermentation of enaminomycins were performed by conventional submerged culture in a 30-liter jar fermentor. Isolation of the antibiotics was performed by centrifugation of the culture broth and adsorption of the antibiotics from the supernatant on a column of activated carbon, followed by elution with aqueous acetone. Enaminomycins A, B and C were separated from each other on a column of Sephadex LH-20.