Transcription of bovine satellite DNA by purified RNA polymerase III

Purified calf thymus RNA polymerase III synthesizes, from calf thymus DNA template, RNA which hybridizes to the major repeated sequence of Eco R1-digested calf thymus DNA. Similar results are obtained with RNA transcribed from calf thymus chromatin. It is suggested that this DNA sequence, which is derived from bovine satellite DNA, may be genetically active.