Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interlaboratory comparison of serum thyroxine analyses

F W Spierto, W H Hannon, D M Fast

    Clinical Biochemistry
    |June 1, 1982
    PubMed
    Summary

    This study surveyed thyroxine (T4) measurements across 300 labs using 35 kits. Abbott and Nuclear Medical kits showed higher T4 values compared to other methods, indicating potential assay variability.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A comparison of results from antihuman globulin-graded reactions with the monocyte monolayer assay.

    Immunohematology·2023
    Same author

    Air Quality Response in China Linked to the 2019 Novel Coronavirus (COVID-19) Lockdown.

    Geophysical research letters·2020
    Same author

    From Watershed to Estuary: Assessment of Nutrient Loading, Retention, and Export from the Ipswich River Basin.

    The Biological bulletin·2017
    Same author

    Performance of succinylacetone assays and their associated proficiency testing outcomes.

    Clinical biochemistry·2012
    Same author

    CFTR mutation analysis and haplotype associations in CF patients.

    Molecular genetics and metabolism·2011
    Same author

    The stability of markers in dried-blood spots for recommended newborn screening disorders in the United States.

    Clinical biochemistry·2011

    Area of Science:

    • Clinical Chemistry
    • Laboratory Medicine
    • Endocrinology

    Background:

    • Thyroxine (T4) is a crucial thyroid hormone.
    • Accurate T4 measurement is vital for diagnosing thyroid disorders.
    • Standardization of T4 assays remains a challenge in clinical laboratories.

    Purpose of the Study:

    • To assess the performance of commercially available thyroxine (T4) measurement kits.
    • To identify variations in T4 measurements among different laboratory methods.
    • To evaluate the precision of T4 assays across various concentration levels.

    Main Methods:

    • Survey of approximately 300 clinical chemistry laboratories.
    • Analysis of T4 concentrations in 13 lyophilized serum specimens.
    • Comparison of results from 35 different commercial T4 assay kits.

    Main Results:

    • Significant variability in T4 measurements was observed among different kits.
    • Abbott and Nuclear Medical kits yielded higher T4 values (1-2 µg/dL) compared to other methods.
    • Within-run coefficients of variation ranged from 10-20% at low T4 levels (<3 µg/dL) and 3-6% at higher levels.

    Conclusions:

    • Commercial T4 assay kits exhibit notable inter-method variability.
    • Specific kits (Abbott, Nuclear Medical) may overestimate T4 concentrations.
    • Assay precision is concentration-dependent, with lower variability at higher T4 levels.

    Related Experiment Videos