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Related Experiment Videos

Isolation of human urinary lysozyme

N Madusa, S Kobayashi, K Mizuno

    Journal of Biochemistry
    |October 1, 1978
    PubMed
    Summary

    A new method efficiently isolates pure human lysozyme from leukemia patient urine using ammonium sulfate precipitation, Sephadex G-50 fractionation, and CM-Sepharose chromatography.

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    Area of Science:

    • Biochemistry
    • Protein purification
    • Clinical chemistry

    Background:

    • Human lysozyme is a key enzyme with diagnostic potential.
    • Leukemia patients exhibit altered urinary protein profiles.
    • Efficient isolation methods for urinary lysozyme are needed.

    Purpose of the Study:

    • To establish a simple and effective method for isolating human lysozyme from the urine of leukemia patients.
    • To characterize the purification process for urinary lysozyme.

    Main Methods:

    • Urinary proteins precipitated using 60% ammonium sulfate saturation.
    • Crude lysozyme fractionated using Sephadex G-50 chromatography.
    • Purification achieved via CM-Sepharose chromatography.

    Main Results:

    • A straightforward purification protocol was developed.
    • Approximately 60% of urinary lysozyme was successfully isolated.
    • The entire process was completed within ten days.

    Conclusions:

    • This method provides a reliable way to obtain pure human lysozyme from leukemia patient urine.
    • The established technique is efficient and time-effective for biochemical and diagnostic applications.

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