Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in pO2 induce retinal autoregulation in vitro

N Papst, E Demant, G Niemeyer

    Graefe'S Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie
    |January 1, 1982
    PubMed
    Summary

    Retinal blood vessels in isolated cat eyes dilated during low oxygen and constricted during high oxygen. However, this autoregulation did not fully maintain stable retinal function, unlike in previous in vivo studies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Force-sensitive detents improve user performance for linear selection tasks.

    IEEE transactions on haptics·2014
    Same author

    The polyethylene glycols.

    Journal of the American Pharmaceutical Association. American Pharmaceutical Association·2010
    Same author

    Syndromic retinitis pigmentosa: ERG and phenotypic changes.

    Klinische Monatsblatter fur Augenheilkunde·2005
    Same author

    Multicenter evaluation of tendency-oriented perimetry (TOP) using the G1 grid.

    European journal of ophthalmology·2003
    Same author

    Retinal research using the perfused mammalian eye.

    Progress in retinal and eye research·2001
    Same author

    [Effect of insulin on retinal glycogen content].

    Klinische Monatsblatter fur Augenheilkunde·2000

    Area of Science:

    • Ophthalmology
    • Physiology
    • Vascular Biology

    Background:

    • Vascular autoregulation is crucial for maintaining stable retinal function despite fluctuations in arterial oxygen partial pressure (pO2).
    • Previous studies in anesthetized cats suggested effective in vivo autoregulation.
    • The autoregulatory capacity of isolated retinal vasculature in response to pO2 changes requires further investigation.

    Purpose of the Study:

    • To investigate the autoregulatory responses of retinal blood vessels in an isolated cat eye model to changes in perfusate pO2.
    • To assess whether autoregulation in vitro adequately compensates for altered oxygen levels to maintain retinal function.

    Main Methods:

    • Isolated cat eyes were perfused under controlled hydrostatic pressure and perfusate composition, with pO2 being the variable.

    Related Experiment Videos

  • Changes in perfusate flow rate were measured during periods of induced hypoxia and hyperoxia.
  • Retinal vasodilation/vasoconstriction was visualized using indocyanine green angiography.
  • Electroretinography (ERG), specifically the b-wave, was used to monitor retinal function and viability.

    • Main Results:

    • Hypoxia (12%-42% pO2 reduction) led to a significant increase in perfusate flow in 8 out of 9 experiments, indicating vasodilation.
    • Hyperoxia (7%-38% pO2 increase) consistently resulted in decreased perfusate flow, indicating vasoconstriction.
    • The b-wave amplitude of the ERG mirrored the changes in perfusate pO2, suggesting incomplete compensation by autoregulation.

    Conclusions:

    • Retinal vessels in the isolated cat eye exhibit autoregulatory vasodilation in response to hypoxia and vasoconstriction in response to hyperoxia.
    • Autoregulation in this in vitro model does not fully compensate for changes in pO2, leading to altered retinal function as evidenced by ERG.
    • These findings contrast with previous in vivo studies, highlighting differences in autoregulatory efficacy between isolated and in situ retinal vasculature.