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Plasma apolipoprotein E levels in hypertriglyceridemia

N Yamada, T Murase, Y Akanuma

    Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
    |June 1, 1982
    PubMed
    Summary
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    A new radioimmunoassay accurately measures human apolipoprotein E (apo E). Elevated apo E levels correlate with higher plasma triglycerides, suggesting a role in their metabolism.

    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Lipid Metabolism

    Background:

    • Apolipoprotein E (apo E) plays a crucial role in lipoprotein metabolism.
    • Accurate measurement of apo E is essential for understanding lipid disorders.

    Purpose of the Study:

    • To develop and validate a specific radioimmunoassay for human apo E.
    • To investigate the relationship between plasma apo E levels and plasma triglyceride concentrations.

    Main Methods:

    • Development of a double-antibody radioimmunoassay using rabbit-raised anti-apo E antibody.
    • Assay validation for specificity and sensitivity.
    • Measurement of apo E in normalipidemic and hypertriglyceridemic subjects.

    Main Results:

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    • The developed radioimmunoassay demonstrated high specificity and sensitivity.
    • Mean plasma apo E in normal subjects was 9.5 +/- 1.8 mg/100 ml.
    • Plasma apo E concentration positively correlated with triglyceride levels in hypertriglyceridemic patients (r=0.84, p<0.001).
    • A negative correlation was observed between the apo E/triglyceride ratio and triglyceride levels (r=0.49, p<0.02).
    • No correlation was found between plasma apo E and cholesterol levels.

    Conclusions:

    • The radioimmunoassay provides a reliable method for quantifying human apo E.
    • Plasma apo E levels are closely associated with plasma triglyceride concentrations.
    • These findings suggest a potential role for apo E in triglyceride metabolism.