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Related Experiment Videos

Lymphokine-induced neutrophil aggregation

P Badenoch-Jones

    Immunology
    |September 1, 1982
    PubMed
    Summary

    Lymphokines (LK) aggregate neutrophils and induce direct neutrophil aggregation in sensitized guinea-pig leukocytes. This aggregation is inhibited by several agents, suggesting specific cellular mechanisms.

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    Area of Science:

    • Immunology
    • Cellular Biology

    Background:

    • Lymphokines (LK) are known to possess macrophage aggregating activity.
    • Neutrophil aggregation is a critical process in inflammatory responses.

    Purpose of the Study:

    • To investigate the effect of lymphokines on neutrophil aggregation.
    • To characterize the nature of neutrophil aggregating activity and its inhibition.

    Main Methods:

    • Purified neutrophils and blood leukocytes were used in aggregation assays.
    • Sephadex fractionation was employed to determine the molecular weight of aggregating factors.
    • Inhibition studies were conducted using various chemical agents.

    Main Results:

    • Lymphokine preparations aggregated purified neutrophils but not mononuclear cells or erythrocytes.
    • Antigen stimulation induced direct aggregation of sensitized guinea-pig leukocytes.
    • Neutrophil aggregation activity was associated with molecular weights of 50,000 and in the 90,000-225,000 range.
    • LK-induced neutrophil aggregation was inhibited by colchicine, prostaglandin E2, chlorpromazine, and glycolytic inhibitors.

    Conclusions:

    • Lymphokines possess direct neutrophil aggregating activity.
    • Neutrophil aggregation can be induced both directly and via soluble factors.
    • The aggregation process involves specific molecular mechanisms sensitive to various inhibitors.

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