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Allomalformin

M Bodanszky, M A Bednarek, A E Yiotakis

    International Journal of Peptide and Protein Research
    |July 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Researchers synthesized allomalformin to explain erroneous malformin sequencing. Allomalformin and malformin share biological activity but differ in chromatography, with allomalformin absent in natural samples. This study clarifies malformin

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    Area of Science:

    • Biochemistry
    • Natural Product Chemistry
    • Peptide Synthesis

    Background:

    • Malformin is a microbial peptide with a complex structure.
    • Previous sequence assignments for malformin were found to be erroneous.
    • A potential sequence isomer, allomalformin, was proposed as a source of error.

    Purpose of the Study:

    • To synthesize and characterize 3-isoleucine-5-valine malformin (allomalformin).
    • To compare allomalformin with natural and synthetic malformin to identify sources of sequencing errors.
    • To elucidate the correct partial sequence of malformin.

    Main Methods:

    • Chemical synthesis of allomalformin.
    • Comparative analysis using biological activity assays, Circular Dichroism (CD), Optical Rotatory Dispersion (ORD), High-Pressure Liquid Chromatography (HPLC), and Thin-Layer Chromatography (TLC).

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  • Partial acid hydrolysis of malformin and its analogs.

    • Main Results:

    • Allomalformin is biologically active and conformationally similar to malformin, but chromatographically distinct.
    • Allomalformin was not detected in natural malformin samples by TLC or HPLC.
    • A lower homolog, 5-valine malformin, was detected in natural samples and identified as the source of misleading hydrolysis fragments (Val-Cys and Val-Ala).

    Conclusions:

    • The erroneous Cys-Val-Cys sequence assignment for malformin likely resulted from the presence of a lower homolog (5-valine malformin).
    • Partial acid hydrolysis of the lower homolog, not malformin itself, generated fragments that led to the incorrect sequence.
    • This study clarifies the structural basis for previous malformin sequencing errors.