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Updated: Aug 10, 2026

Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons
Published on: December 10, 2009
Recombinant DNA methods in neuroendocrinology: new answers to old questions
Abstract:
The development of methods for cloning DNA complementary to specific messenger RNAs encoding polypeptide hormones has opened new experimental avenues in the neurosciences. Recombinant DNA techniques have been used to answer questions about the structure of polypeptide hormone precursors, the regulation of peptide hormone production, and the location of peptide-producing cells in nervous and endocrine systems. Here, the application of cDNA cloning to the quantitation, structural elucidation and tissue localization of mRNAs coding for neuroendocrine peptides is reviewed.
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