Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Formulae for maximum valid dilution

J F Cooper

    Progress in Clinical and Biological Research
    |January 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    The MVD calculation offers a reliable method for comparing in vitro and in vivo endotoxin tests. Clearer labeling of Limulus amebocyte lysate (LAL) reagents and endotoxin standards is crucial for the parenteral industry.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    MeV proton flux predictions near Saturn's D ring.

    Journal of geophysical research. Space physics·2016
    Same author

    Methylmercuric chloride induces activation of neuronal stress circuitry and alters exploratory behavior in the mouse.

    Neuroscience·2007
    Same author

    An investigation of the spatial variations of the energetic trapped electrons at low altitudes.

    Advances in space research : the official journal of the Committee on Space Research (COSPAR)·2001
    Same author

    Kinetics of chemical degradation of isoxaflutole: influence of the nature of aqueous buffers (alkanoic acid/sodium salt vs phosphate).

    Pest management science·2001
    Same author

    Fate of a phenylpyrazole in vegetation and soil under tropical field conditions.

    Journal of agricultural and food chemistry·2001
    Same author

    Kinetics of abiotic hydrolysis of isoxaflutole: influence of pH and temperature in aqueous mineral buffered solutions.

    Journal of agricultural and food chemistry·2000
    Same journal

    DNA homology and chromosome stability: a sensitive yeast genetic system for identifying double-stranded DNA damage.

    Progress in clinical and biological research·2018
    Same journal

    Endotoxin and sepsis: molecular mechanisms of pathogenesis, host resistance, and therapy. Proceedings of the 4th Conference of the International Endotoxin Society. Nagoya, Japan, October 23-27, 1996.

    Progress in clinical and biological research·1998
    Same journal

    The pathogenic role of LBP in gram-negative sepsis and septic shock.

    Progress in clinical and biological research·1998
    Same journal

    The role of interleukin 6 in endotoxin-induced inflammatory responses.

    Progress in clinical and biological research·1998
    Same journal

    Role of nitric oxide and reactive oxygen species in endotoxin shock.

    Progress in clinical and biological research·1998
    Same journal

    Interaction of lipopolysaccharide with a mammalian lyso-phosphatidate acyltransferase (LPAAT) transfected into E. coli, and effect of lisofylline on LPAAT transfected into mammalian cells.

    Progress in clinical and biological research·1998
    See all related articles

    Area of Science:

    • Microbiology
    • Biotechnology
    • Pharmaceutical Science

    Background:

    • Endotoxin testing is critical for parenteral drug safety.
    • Current labeling of Limulus amebocyte lysate (LAL) reagents and control standard endotoxins causes confusion.
    • Discrepancies exist between in vitro and in vivo endotoxin detection methods.

    Purpose of the Study:

    • To evaluate the MVD calculation as a method for equating in vitro and in vivo endotoxin test sensitivity.
    • To highlight the need for standardized and clear labeling of endotoxin testing materials.
    • To assess the safety margin provided by the LAL reagent's reactivity compared to traditional bioassays.

    Main Methods:

    • Preparation of test specimens using the MVD (Mathematical Volume Determination) calculation.

    Related Experiment Videos

  • Analysis of LAL reagent reactivity to environmental endotoxin.
  • Comparison with rabbit bioassay sensitivity.

    • Main Results:

    • The MVD calculation provides a reasonable approach to equating in vitro and in vivo endotoxin test sensitivity.
    • LAL reagent demonstrates greater reactivity to environmental endotoxin than the rabbit bioassay.
    • This enhanced reactivity offers a greater safety margin for the in vitro testing approach.

    Conclusions:

    • Standardized, explicit labeling of LAL reagents and endotoxin standards in EU/ml and ng/ml is essential.
    • Government and reagent suppliers must collaborate to provide unambiguous test materials.
    • The increased sensitivity of LAL testing suggests that rabbit retesting may be suitable for specific product types following LAL test failures.