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Related Experiment Videos

Simple colorimetric method for seminal plasma zinc assay

J Fuentes, J Miró, J Riera

    Andrologia
    |July 1, 1982
    PubMed
    Summary

    This study presents a simple colorimetric method for measuring seminal plasma zinc, a key indicator of male reproductive health. The new assay simplifies zinc analysis, overcoming technical challenges in many labs.

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    Area of Science:

    • Reproductive Biology
    • Clinical Chemistry
    • Analytical Chemistry

    Background:

    • Seminal plasma zinc concentration is a valuable biomarker for assessing male prostatic function and diagnosing infertility.
    • Current methods for zinc analysis in seminal plasma present technical difficulties, limiting widespread laboratory use.

    Purpose of the Study:

    • To develop and validate a simple, accurate, and interference-free colorimetric method for quantifying zinc in seminal plasma.
    • To provide an accessible assay for routine clinical use in male infertility diagnostics.

    Main Methods:

    • A novel colorimetric assay utilizing 1-(2-pyridylazo)-2-naphthol and dodecyl hydrogen sulfate sodium salt.
    • The method involves a single reagent solution, eliminating the need for deproteinization and organic solvent extraction.
    • Absorbance is measured at 560 nm for the colored zinc complex.

    Main Results:

    • The developed colorimetric method demonstrated accuracy comparable to atomic absorption spectrophotometry.
    • The assay exhibited good precision and was found to be free from significant interference.
    • The simplified procedure removes technical barriers associated with traditional zinc assays.

    Conclusions:

    • This new colorimetric method offers a practical and reliable approach for seminal plasma zinc determination.
    • The assay facilitates the routine assessment of prostatic function in male infertility evaluations.
    • The method's simplicity and accuracy make it suitable for broader clinical laboratory application.

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