Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Self-association of daunomycin

J B Chaires, N Dattagupta, D M Crothers

    Biochemistry
    |August 17, 1982
    PubMed
    Summary

    Daunomycin, an anthracycline antibiotic, self-associates in solution. This indefinite association, characterized by NMR and other methods, is minimal under typical drug-DNA binding study conditions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Thermodynamic characterization of human telomere quadruplex unfolding.

    Biopolymers·2013
    Same author

    Targeting DNA.

    Biochimie·2011
    Same author

    Climate change and the integrity of science.

    Science (New York, N.Y.)·2010
    Same author

    RNA folding pathways.

    Current protocols in nucleic acid chemistry·2008
    Same author

    Binding of netropsin to several DNA constructs: evidence for at least two different 1:1 complexes formed from an -AATT-containing ds-DNA construct and a single minor groove binding ligand.

    Biophysical chemistry·2006
    Same author

    Comparison of analyses of DNA curvature.

    Journal of biomolecular structure & dynamics·2003

    Area of Science:

    • Biochemistry
    • Chemical Physics

    Background:

    • Daunomycin is a potent anthracycline antibiotic.
    • Anthracyclines are known for their DNA-binding properties, crucial in cancer therapy.
    • Understanding drug self-association is vital for accurate drug-DNA interaction studies.

    Purpose of the Study:

    • To characterize the self-association behavior of daunomycin in aqueous solution.
    • To determine the model and equilibrium constant governing daunomycin self-association.
    • To assess the impact of daunomycin aggregation on drug-DNA binding studies.

    Main Methods:

    • Visible absorbance spectroscopy
    • Sedimentation equilibrium ultracentrifugation
    • Proton nuclear magnetic resonance (NMR) spectroscopy

    Main Results:

    • Daunomycin self-associates at concentrations above 10 microM.
    • An indefinite association model, not simple dimerization, best describes the aggregation.
    • An intrinsic association constant (Ki) of 1500 M-1 and an enthalpy of -8.0 kcal/mol were determined.
    • NMR data indicate aggregation involves stacking of the anthracycline rings.

    Conclusions:

    • The self-association of daunomycin follows an indefinite association model.
    • Under common experimental conditions for drug-DNA binding, daunomycin aggregation is negligible.
    • The findings allow for more accurate interpretation of daunomycin-DNA binding data.

    Related Experiment Videos