[Calculation of the surface and volume of proteins in solution]

The volume occupied by the particle in the solvent and also its molecular and accessible surfaces were described with a system of small cubes (the edge length approximately 0.3 A). We have compared the ratios between molecular and accessible surface areas for a number of proteins, and different extent of jaggedness of the molecular surface of these proteins was revealed. For the studied proteins the volume occupied by the protein in the solvent calculated by this technique is close to that estimated from the experimental partial specific protein volume.