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RNA-reverse transcriptase complex from cultured human myeloma-leukemia cells

H Sawada, M Tashima, T Nakamura

    International Journal of Cancer
    |July 15, 1977
    PubMed
    Summary
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    Researchers detected a high molecular weight RNA-reverse transcriptase complex in patients with myeloma-leukemia. This complex, found in leukocyte culture media, shows significant DNA polymerizing activity.

    Area of Science:

    • * Molecular biology
    • * Virology
    • * Oncology

    Background:

    • * Myeloma-leukemia is a complex hematological malignancy.
    • * Peripheral leukocytes are key immune cells.
    • * RNA-reverse transcriptase complexes are associated with retroviruses and cellular genetic elements.

    Observation:

    • * A high molecular weight RNA-reverse transcriptase complex was identified in the culture media of peripheral leukocytes from two Japanese myeloma-leukemia patients.
    • * This complex exhibited a 3H-uridine peak and DNA polymerizing activity.
    • * The complex banded at a density of 1.15-1.19 g/ml.

    Findings:

    • * The enzyme within the complex demonstrated RNA-dependent DNA polymerase activity, utilizing poly(rA)-d(pT)10 and poly(rC)-d(pG)12-18 as template-primers.

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  • * It did not utilize poly(dA)-d(pT)10 or (dT)12-18, indicating a preference for RNA templates.
  • * Sucrose density sedimentation revealed RNA components sedimenting at approximately 50s and 20-30s.

    • Implications:

    • * The presence of this complex suggests potential viral involvement or novel endogenous retroviral activity in myeloma-leukemia.
    • * Further characterization could reveal new diagnostic markers or therapeutic targets.
    • * This finding contributes to understanding the molecular mechanisms underlying myeloma-leukemia pathogenesis.