Effects of duct ligation and orchidectomy on histochemical reactions in the hamster epididymis

The epididymis was ligated at the efferent ductules and at the middle segment. Histochemical changes occurred only proximal to the ligature. Orchidectomy caused progressive changes, mostly reversible by testosterone treatment throughout the epididymis. It is concluded that the integrity of the initial segment is dependent upon androgen reaching it via the testicular fluid.