Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence organization of rat brain mitochondrial DNA

R Meeker, V Tandon, K Oishi

    Brain Research
    |August 12, 1982
    PubMed
    Summary

    Rat brain and liver mitochondrial DNA (mtDNA) sequences are identical, showing no heterogeneity. Analysis confirmed consistent DNA fragments and no methylated cytosine residues in either tissue.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Estimation of the energy expenditure of grazing ruminants by incorporating dynamic body acceleration into a conventional energy requirement system.

    Journal of animal science·2017
    Same author

    Risk assessment of recent Egyptian H5N1 influenza viruses.

    Scientific reports·2016
    Same author

    Comprehensive population screening in the Ashkenazi Jewish population for recurrent disease-causing variants.

    Clinical genetics·2016
    Same author

    Accumulation of Brain Tryptophan in Rats after Administering Various Fats or Fatty Acids.

    Bioscience, biotechnology, and biochemistry·2016
    Same author

    Successful control of an outbreak of GES-5 extended-spectrum β-lactamase-producing Pseudomonas aeruginosa in a long-term care facility in Japan.

    The Journal of hospital infection·2016
    Same author

    Factors influencing the priority of access to food and their effects on the carcass traits for Japanese Black (Wagyu) cattle.

    Animal : an international journal of animal bioscience·2015

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • Mitochondrial DNA (mtDNA) plays a crucial role in cellular respiration and energy production.
    • Understanding the sequence organization of mtDNA is essential for studying genetic variation and evolutionary relationships.
    • Rat mtDNA serves as a model system for investigating mitochondrial genetics in mammals.

    Purpose of the Study:

    • To characterize the sequence organization of rat brain and liver mitochondrial DNA.
    • To investigate potential heterogeneity in mtDNA sequences between different rat tissues.
    • To confirm the presence and integrity of mtDNA fragments within recombinant DNA molecules.

    Main Methods:

    • Restriction endonuclease digestion (HindIII, BamHI, HhaI, HaeIII, HpaII/MspI) of rat brain and liver mtDNA.
    • Analysis of DNA fragments by gel electrophoresis and molecular size determination.
    • Construction and analysis of recombinant DNA molecules containing mtDNA fragments using pBR322.
    • Electron microscopy and Southern blot hybridization to confirm fragment integrity and presence.

    Main Results:

    • Rat brain and liver mtDNAs produced identical DNA fragment patterns when digested with restriction enzymes, indicating no sequence heterogeneity.
    • Enzymatic digestion with HpaII/MspI revealed no evidence of methylated cytosine residues in either mtDNA.
    • Recombinant DNA molecules successfully incorporated all identified mtDNA fragments, with sizes consistent with electron microscopy and molecular weight analyses.
    • Southern blot hybridization confirmed the presence of mtDNA fragments within the recombinant DNA molecules.

    Conclusions:

    • Rat brain and liver mitochondrial DNA sequences are highly conserved and exhibit no detectable heterogeneity at the base sequence level.
    • The characterized mtDNA fragments were accurately represented in the constructed recombinant DNA molecules.
    • This study provides a detailed molecular map of rat mtDNA, valuable for future genetic and functional studies.

    Related Experiment Videos