Participation of corticothalamic cells in penicillin-induced generalized spike and wave discharges

Single unit extracellular recordings were performed in the cortex of awake painlessly immobilized unanesthetized cats during generalized spike and wave discharges (SW) induced by i.m. penicillin. Corticothalamic cells were identified in cortical areas 3a and 4 gamma by stimulating n. ventralis lateralis (VL) and in cortical areas 5 and 7 by stimulating n. lateralis posterior (LP). Twelve of 24 neurons antidromically invaded from VL were also pyramidal tract cells. Two of 11 neurons antidromically invaded from LP also displayed orthodromic responses. Corticothalamic cells fired bursts of action potentials in association with the 'spike' whereas a period of inhibition was associated with the 'wave' of the SW complex. The data suggest that in this experimental model the appearance of SW in the thalamus is due to secondary activation of thalamic neurons by volleys arising from the cortex and mediated through corticothalamic connections.