Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An assay for creatine kinase conversion factor

H Falter, L Michelutti, A Mazzuchin

    Clinical Biochemistry
    |August 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Altered blood chemistry and hippocampal histomorphology in adult rats following prenatal exposure to physiologically-patterned, weak (50-500 nanoTesla range) magnetic fields.

    International journal of radiation biology·2008
    Same author

    Effects of tetrodotoxin-induced neural inactivation on single muscle fiber metabolic enzymes.

    The American journal of physiology·1994
    Same author

    Behaviors of rats with insidious, multifocal brain damage induced by seizures following single peripheral injections of lithium and pilocarpine.

    Physiology & behavior·1993
    Same author

    Transient suppression of a secondary humoral response in rats is evoked by lithium-pilocarpine-induced limbic seizures.

    Pharmacology, biochemistry, and behavior·1992
    Same author

    Infantile stimulation produces mild enhancement in a primary humoral response of adult albino rats.

    Psychological reports·1992
    Same author

    Gallium toxicity and adaptation in Pseudomonas fluorescens.

    FEMS microbiology letters·1992

    Researchers developed a new assay to measure creatine kinase conversion factor, an enzyme modifying creatine kinase (CK) isoenzymes. This assay quantifies the rate of MM3 to MM1/MM2 conversion in human serum.

    Area of Science:

    • Biochemistry
    • Enzymology
    • Clinical Chemistry

    Background:

    • Creatine kinase (CK) isoenzymes, specifically the M subunits (MM and MB), undergo postsynthetic modification in serum.
    • This modification results in three MM sub-bands (MM3, MM2, MM1) with distinct electrophoretic mobilities detectable by agarose electrophoresis.

    Purpose of the Study:

    • To develop and characterize an assay for creatine kinase conversion factor.
    • To quantify the rate of conversion of MM3 to MM2 and MM1 sub-bands.

    Main Methods:

    • Development of an assay measuring the catalytic activity of creatine kinase conversion factor.
    • Assay based on the rate of conversion of MM3 to MM2 and MM1.
    • Agarose electrophoresis used to identify and differentiate MM sub-bands.

    Related Experiment Videos

    Main Results:

    • The assay quantifies the first-order rate constant (kc) for the MM3 to MM2/MM1 conversion.
    • For 24 healthy adults, kc values ranged from 0.0221 to 0.0740 hr-1, with a mean of 0.0392 hr-1.
    • The temperature and pH dependence of kc were determined.

    Conclusions:

    • A reliable assay for creatine kinase conversion factor has been established.
    • The assay provides a quantitative measure of CK isoenzyme postsynthetic modification.
    • Further studies can explore the clinical significance of creatine kinase conversion factor levels.