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Four methods for determining uric acid compared with a candidate reference method

R J Elin, E Johnson, R Chesler

    Clinical Chemistry
    |October 1, 1982
    PubMed
    Summary
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    Comparing serum uric acid measurement methods, the aca method showed the least deviation from the reference method. The SMAC phosphotungstic acid method had the closest slope to unity for uric acid analysis.

    Area of Science:

    • Clinical Chemistry
    • Biochemical Analysis
    • Analytical Methodology

    Background:

    • Accurate measurement of serum uric acid is crucial for diagnosing and managing conditions like gout and kidney disease.
    • Several analytical methods exist for quantifying serum uric acid, each with potential variations in accuracy and precision.
    • Standardization and validation of uric acid assays against a reference method are essential for reliable clinical laboratory results.

    Purpose of the Study:

    • To compare the performance of four commonly used serum uric acid assay methods against a candidate Reference Method.
    • To evaluate the accuracy and agreement of uricase-based (aca, Ektachem, SMAC) and phosphotungstic acid-based (SMAC) methods for uric acid determination.
    • To identify the most reliable method for serum uric acid measurement in a clinical laboratory setting.

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    Main Methods:

    • Serum specimens from 83 patients with varying uric acid levels (19–141 mg/L) were analyzed.
    • Five methods were employed: three uricase methods (aca, Ektachem, SMAC) and one phosphotungstic acid SMAC method, compared to a candidate Reference Method.
    • Linear regression analysis and calculation of mean differences were used to compare results between methods.

    Main Results:

    • The aca method exhibited the smallest intercept deviation and mean difference compared to the Reference Method.
    • The SMAC phosphotungstic acid method demonstrated a slope closest to unity when compared to the Reference Method.
    • The SMAC uricase method showed the largest intercept deviation and greatest slope deviation from unity.

    Conclusions:

    • The aca method appears to be a highly accurate and reliable option for serum uric acid measurement.
    • The SMAC phosphotungstic acid method offers good agreement with the reference standard, particularly in terms of slope.
    • Method selection for serum uric acid analysis should consider accuracy, agreement with reference standards, and potential biases.