Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A calibration protocol for serum-based secondary standards

W G Miller, D J Rhodes, C J Moore

    Clinical Chemistry
    |November 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Black Hole Spectroscopy and Tests of General Relativity with GW250114.

    Physical review letters·2026
    Same author

    GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

    Physical review letters·2025
    Same author

    Search for Subsolar-Mass Binaries in the First Half of Advanced LIGO's and Advanced Virgo's Third Observing Run.

    Physical review letters·2022
    Same author

    Constraints on Cosmic Strings Using Data from the Third Advanced LIGO-Virgo Observing Run.

    Physical review letters·2021
    Same author

    Prospects for observing and localizing gravitational-wave transients with Advanced LIGO, Advanced Virgo and KAGRA.

    Living reviews in relativity·2020
    Same author

    GW190521: A Binary Black Hole Merger with a Total Mass of 150  M_{⊙}.

    Physical review letters·2020
    Same journal

    Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

    Clinical chemistry·2026
    Same journal

    Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

    Clinical chemistry·2026
    Same journal

    TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

    Clinical chemistry·2026
    Same journal

    Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

    Clinical chemistry·2026
    Same journal

    Editor's Note: Circulating Proteolytic Products of Carboxypeptidase N for Early Detection of Breast Cancer.

    Clinical chemistry·2026
    Same journal

    In Reply to Reflexing NT-proBNP for sFlt-1/PlGF Ratios That Fall into the Measurement Uncertainty for Preeclampsia Risk Classification.

    Clinical chemistry·2026
    See all related articles

    This study introduces a new calibration method for clinical chemistry assays. It ensures accurate patient results by linking secondary standards to primary standards, correcting for serum matrix interference.

    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Laboratory Medicine

    Background:

    • Accurate calibration is crucial for reliable clinical laboratory results.
    • Serum-based secondary standards can introduce matrix interference, affecting accuracy.
    • Existing calibration methods may not adequately address these interferences.

    Purpose of the Study:

    • To develop and validate a novel calibration procedure for clinical chemistry assays.
    • To correct for matrix interference in serum-based secondary standards.
    • To ensure accurate patient-sera results by linking to primary standards.

    Main Methods:

    • Tentative calibration values assigned to secondary standards using routine instruments.
    • Patient serum samples analyzed by both routine and primary standard calibration methods.

    Related Experiment Videos

  • Adjustment of secondary standard calibration values to achieve agreement between methods.

    • Main Results:

    • The developed procedure successfully linked routine method calibration to primary standards.
    • Significant differences observed between determined and manufacturer-assigned secondary standard values for multiple analytes.
    • The method demonstrated effectiveness in correcting for serum matrix interference.

    Conclusions:

    • The proposed calibration method provides accurate results for patient sera by addressing matrix interference.
    • This approach enhances the reliability of clinical chemistry assays.
    • The findings suggest a need to re-evaluate manufacturer-assigned calibration values for secondary standards.